SRP6007

NT-3 from mouse

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)

• Synonym(s): HDNF, NGF-2, NT-3, Nerve growth factor-2, Neurotrophic factor
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• Assay: ≥97% (HPLC); ≥97% (SDS-PAGE)
• form: lyophilized
• mol wt: 13.6 kDa
• packaging: pkg of 10 μg
• impurities: Endotoxin, tested
• NCBI accession no.: P20181
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... NTF3(18205)

Description

General description

NT-3 (neurotrophin-3) is a member of the family of trophic factors, called neurotrophins. This family also includes NGF (nerve growth factor), BDNF (brain-derived neurotrophic factor), and NT-4. NT-3 is the most abundantly expressed neurotrophin during early development.
Murine NT-3 produced from Escherichia coli contains 119 amino acid and having a molecular mass is 13.6kDa.

Application

NT-3 (neurotrophin-3) has been used for the ex vivo treatment of skin punches obtained from rat and human samples.

Biochem/physiol Actions

Study in mice show that NT-3 (neurotrophin-3), along with BDNF (brain-derived neurotrohpic factor), is essential for inner ear development. In vitro motor, enteric, primary sensory, sympathetic, locus coeruleus, cerebellar and hippocampal neurons are responsive to NT-3. Mice null for NT-3 show significant deficiency in sympathetic and sensory nerve populations. In mice, post birth up-regulation of NT-3 induces a second wave of dopaminergic amacrine cell genesis.

Physical form

Sterile filtered and lyophilized powder

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in H?O to a concentration of 0.1-1.0 mg/ml.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable