RAB0543

Human IL18 / Interleukin-18 ELISA Kit

for serum, plasma, cell culture supernatants and urine

• Synonym(s): IFN-gamma-inducing factor, IL-18, Interleukin-18
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... IL18(3606)
• Input: sample type cell culture supernatant(s); sample type urine; sample type plasma; sample type serum
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable
• input: sample type cell culture supernatant(s); sample type urine; sample type plasma; sample type serum
• assay range: inter-assay cv: <10%; intra-assay cv: <12%; sensitivity: 0.5 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL18(3606)

Description

General description

The antibody pair provided in this kit detects Human IL-18 in serum, plasma, cell culture supernatants, and urine. Interleukin-18 (IL-18) is a proinflammatory cytokine, expressed in most of the mammalian tissues including adipose, liver and skeletal muscle. It is generated as pro-IL-18 which undergoes cleavage to form a mature IL-18 possessing the signal peptide. IL-18 induces Th1 (T helper cell 1) lymphocyte action mediated by interferon-γ. It controls the metabolism of lipids and glucose. Inflammasome activation is responsible for IL-18 release, which is a significant event in response to viral infection. Increased level of IL-18 is observed in kidney and liver disease, diabetes, atherosclerosis, autoimmune disease such as rheumatoid arthritis and asthma. It is also associated with the development of renal dysfunction.

Application

Human IL18 / Interleukin-18 ELISA Kit has been used to evaluate the macrophage cytokines production in response to trans-stilbene and terphenyl compounds. It has also been used to analyze and measure IL-18 levels in the urine of chronic kidney disease patients.

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDS
• RABELADA

  ELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable