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RAB0210

Mouse Granzyme B ELISA Kit

for serum, plasma and cell culture supernatant

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1 kit
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
$721.00

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Gzmb(14939)
Input:
sample type plasma
sample type serum
sample type cell culture supernatant(s)
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice

$721.00


Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)

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species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 14.34-3500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Gzmb(14939)

General description

The Mouse Granzyme B ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Granzyme B in serum, plasma, cell culture supernatants and urine.
The Mouse Granzyme B enzyme-linked immunosorbent assay (ELISA) kit is an in vitro assay for the quantitative measurement of mouse granzyme B in serum, plasma, cell culture supernatants and urine. Granzyme B binds to mannose-6-phosphate/insulin-like growth factor II receptor. This protein is implicated in fast DNA fragmentation, a key step towards apoptosis in cells. Granzyme B is involved in the cleaving of pro-caspase-3, which leads to the activation of caspase-3. This protein also cleaves caspase-6, -7, -8, -9, and -10.[1]

Immunogen

Recombinant Mouse Granzyme B

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB0338RAB0510RAB0125
species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

Gene Information

mouse ... Gzmb(14939)

Gene Information

mouse ... Lgals7(16858)

Gene Information

mouse ... Vegfa(22339)

Gene Information

mouse ... Cxcl12(20315)

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type cell lysate
sample type tissue lysate

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 14.34-3500 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 20.48-5000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 4.1-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 9.38-600 pg/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids



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Questions

  1. It says here that this ELISA kit works for the detection of GzmB in mouse serum, but I can't find any papers that have used serum. Could you please cite a paper or previous work demonstrating the utility of this kit for detecting GzmB in serum?

    1 answer
    1. Unfortunately, published citations on the use of this kit with mouse serum are not available. Please see the image below for an example of expected recovery rates:

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