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P7306

Polyethylene glycol/ dimethyl sulfoxide solution

Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

Synonym(s):

PEG/DMSO Solution, Polyethylene Glycol Solution, Solution of PEG and DMSO

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5 x 5 mL
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$116.00

About This Item

MDL number:
MFCD00081839
UNSPSC Code:
12161902
PubChem Substance ID:
24898839
NACRES:
NA.75

$116.00

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grade

Hybri-Max™

Quality Segment

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

mol wt

average mol wt 1,450

concentration

50 % (w/v)

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

Application

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.


Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.

Packaging

Packaged in sealed ampules under nitrogen.

Preparation Note

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

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Polyethylene glycol/ dimethyl sulfoxide solution Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

Sigma-Aldrich

P7306

Polyethylene glycol/ dimethyl sulfoxide solution

Polyethylene glycol solution Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

Sigma-Aldrich

P7181

Polyethylene glycol solution

Polyethylene glycol solution 40 % (w/w) in H2O, average mol wt 8,000

Sigma-Aldrich

P1458

Polyethylene glycol solution

Polyethylene glycol solution BioUltra, Molecular Biology, 1,000, ~50% in H2O

Sigma-Aldrich

76293

Polyethylene glycol solution

form

solution

form

solution

form

-

form

liquid

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

mol wt

average mol wt 1,450

mol wt

average mol wt 1,450

mol wt

average mol wt 8,000

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, none detected, phosphatases, none detected, proteases, none detected

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

-

technique(s)

-

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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