For products requiring long-term storage in a freezer, labels indicate -20 °C. Typically, freezer facilities used for storing these products are thermostatically controlled to maintain temperatures within -10 to -25 °C. This range aligns with the recommended freezer storage conditions indicated in the United States Pharmacopeia.
P0609
Pepsin−Agarose from porcine gastric mucosa
lyophilized powder, ≥30 units/mg dry solid
Synonym(s):
Pepsin A
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| Size/SKU | Availability | Price |
|---|---|---|
10000 units | Available to ship TODAYfromMILWAUKEE | $285.00 |
25000 units | Available to ship TODAYfromMILWAUKEE | $577.00 $490.45 |
50000 units | Available to ship TODAYfromMILWAUKEE | $994.00 |
About This Item
Specific activity:
≥30 units/mg dry solid
Biological source:
Porcine gastric mucosa
$285.00
Available to ship TODAYDetails
biological source
Porcine gastric mucosa
Quality Segment
form
lyophilized powder
specific activity
≥30 units/mg dry solid
mol wt
35 kDa
matrix
4% cross-linked beaded agarose
UniProt accession no.
storage temp.
−20°C
Gene Information
pig ... LOC396892(396892)
General description
Pepsin is an enzyme found in gastric secretion. [1]
Application
Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin. [2]
Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.
Used to produce F(ab′)2 fragments of antibodies.1
Biochem/physiol Actions
Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.
Physical form
Lyophilized powder stabilized with lactose
Preparation Note
One gram solid swells to 5-10ml packed gel.
Other Notes
One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) | Gene Information pig ... LOC396892(396892) |
| specific activity ≥30 units/mg dry solid | specific activity ≥400 units/mg protein | specific activity ≥2,500 units/mg protein (E1%/280) | specific activity ≥250 units/mg solid |
| biological source Porcine gastric mucosa | biological source Porcine gastric mucosa | biological source - | biological source Porcine gastric mucosa |
| form lyophilized powder | form powder | form lyophilized powder | form powder |
| mol wt 35 kDa | mol wt 35 kDa | mol wt 35 kDa | mol wt 35 kDa |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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It is impossible to store a reagent at -20. freezer's have an operating window of a few degrees either side of the temperature it is set at therefore it is not an obtainable storage requirement. please can you advise a range that is suitable for storage.
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