MAK515
Oxaloacetate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Oxaloacetate Quantification Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
Quality Level
detection method
colorimetric
fluorometric
relevant disease(s)
cancer; neurological disorders
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate.
Features and Benefits
- Linear detection range: 7 to 400 µM for the colorimetric assay and 1 to 40 µM for the fluorometric assay.
Suitability
The kit is suitable for oxaloacetate determination in plasma, serum, tissue and culture media.
Principle
The Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the oxaloacetate concentration in the sample.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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