We have not tested the product in the above mentioned application. The product has been tested to be suitable for a variety of biological samples (e.g., plasma, serum, urine, tissue, and culture media.) or food/beverage samples (e.g., wine, coffee, and juice). The cell pellet may not be suitable. To discuss this further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
MAK321
Acetaldehyde Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
Acetaldehyde Quantitation Kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $388.00 |
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 fluorometric tests
input
culture(s)
food(s)
serum
plasma
tissue
urine
beverage(s)
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
gastrointestinal diseases
storage temp.
−20°C
General description
Acetaldehyde is one of the most widely occurring aldehydes in nature and commonly used in industry. The metabolic byproduct of ethanol in the liver, acetaldehyde is toxic to the human body and rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing known as alcohol flush reaction. Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine.
Application
Suitable for biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice)
The Acetaldehyde Assay Kit may be used for:
- Food and Beverage Testing
- Gastrointestinal Disease Research
Biochem/physiol Actions
The fluorimetric acetaldehyde assay is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the generated NADH reduces a probe making it fluorescent. The fluorescence intensity of the product measured at λex/em = 530/585 nm is directly proportional to acetaldehyde concentration in the sample.
Features and Benefits
Enhanced Detection Range: Detect Acetaldehyde levels accurately across a wide range, from 0.5 µM to 60 µM, using only 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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This Item | |||
|---|---|---|---|
| detection method fluorometric | detection method fluorometric | detection method colorimetric | detection method colorimetric, fluorometric |
| usage sufficient for 100 fluorometric tests | usage sufficient for 200 fluorometric tests | usage - | usage sufficient for 100 colorimetric or fluorometric tests |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| input culture(s) | input - | input cell culture medium | input biological sample(s) |
| application(s) cosmetics | application(s) environmental | application(s) agriculture | application(s) pharmaceutical |
| relevant disease(s) gastrointestinal diseases | relevant disease(s) neurological disorders | relevant disease(s) gastrointestinal diseases | relevant disease(s) gastrointestinal diseases; cancer |
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Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
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Hi, I want to ask a question regarding the fluorometric acetaldehyde assay kit (MAK321). Is it suitable for determining acetaldehyde levels in cell culture pellets, and what is the detection range if cell culture supernatant is used?
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