Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

MAK136

Monoamine Oxidase Activity Assay Kit

sufficient for 100 fluorometric tests

Monoamine Oxidase Activity Assay Kit

Synonym(s):

MAO Assay Kit

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
1 kit
Check Cart for Availability
$522.00

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

$522.00

Check Cart for Availability
​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

neurological disorders

storage temp.

−20°C

Gene Information

human ... MAOA(4128), MAOB(4129)
mouse ... MAOA(17161), MAOB(109731)
rat ... MAOA(29253), MAOB(25750)

General description

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. In mammals, MAO consists of two isoenzymes, MAO-A and MAO-B. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAO in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.

Application

Monoamine Oxidase Activity Assay Kit has been used to estimate MAO-A (monoamine oxidase A), MAO-B (monoamine oxidase B) and ALDH (aldehyde dehydrogenase) activities.
Suitable for the determination of MAO activity in biological samples and for the screening of MAO inhibitors. Can determine either MAO-A, MAO-B or both MAO-A and MAO-B activity.

Biochem/physiol Actions

The Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity in biological samples. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (λex = 530/λem = 585 nm). The assay is simple, sensitive, stable, and high-throughput adaptable. Unit definition: one unit of MAO catalyzes the formation of 1 μmole of H2O2 per minute under the assay conditions.

Features and Benefits

Compatible with high-throughput handling systems.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
SAB5701505M7441AV43557
Monoamine Oxidase Activity Assay Kit sufficient for 100 fluorometric tests

Sigma-Aldrich

MAK136

Monoamine Oxidase Activity Assay Kit

Anti-Monoamine Oxidase A (MAOA) antibody produced in rabbit

Sigma-Aldrich

SAB5701505

Anti-Monoamine Oxidase A (MAOA) antibody produced in rabbit

Monoamine Oxidase B human recombinant, expressed in baculovirus infected BTI insect cells

Sigma-Aldrich

M7441

Monoamine Oxidase B human

Anti-MAOB antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

AV43557

Anti-MAOB antibody produced in rabbit

Gene Information

human ... MAOA(4128), MAOB(4129)
mouse ... MAOA(17161), MAOB(109731)
rat ... MAOA(29253), MAOB(25750)

Gene Information

human ... MAOA(4128)

Gene Information

human ... MAOB(4129)

Gene Information

human ... MAOB(4129)

detection method

fluorometric

detection method

-

detection method

-

detection method

-

usage

sufficient for 100 fluorometric tests

usage

-

usage

-

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

relevant disease(s)

neurological disorders

relevant disease(s)

-

relevant disease(s)

-

relevant disease(s)

-

Storage Class

10 - Combustible liquids

wgk

WGK 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

1 Review This action will navigate to reviews.

Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the concentration of the Dye Reagent stock that is included in the kit?

    1 answer

    1. The Dye Reagent that is provided as a component of this kit is a solution in Dimethyl Sulfoxide (DMSO). This product is made by a third party; the original manufacturer holds information on the identity and concentration of the dye, as well as similar information on other components of the kit, as proprietary.

      Helpful?

  4. What is the concentration of the p-tyramine stock and the HRP enzyme that is included in the kit?

    1 answer

    1. The concentration of the p-tyramine included in the kit is 100mM, and the enzyme concentration is 0.02 U/uL.

      Helpful?

  5. Is it possible to determine if this kit would be suitable for the quantitation of MAO-N?

    1 answer

    1. This kit is not tested with MAO-N. It is important to note that this kit was specifically developed with MAO-A and MAO-B in mind, and it is uncertain whether other enzymes, such as MAO-N, would be compatible with the substrate.

      Helpful?

Reviews

Active Filters

  1. New York, NY
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Performed Flawlessly

    This has produced consistent results every single time.

    Helpful?