G5768

Gamborg′s B-5 Basal Salt Mixture

fine powder, suitable for plant cell culture

• Synonym(s): B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium
• NACRES: NA.72
• UNSPSC Code: 12352207

Properties

• form: fine powder
• Quality Segment: 200
• technique(s): cell culture | plant: suitable
• application(s): agriculture
• storage temp.: 2-8°C

Description

General description

Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Application

Gamborg′s B-5 Basal Salt Mixture has been used:

• as a constituent during the preparation of TEX buffer
• as a media to maintain potato suspension and callus cultures
• as a media in the primary-node transformation method

Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Preparation Note

Formulated to contain 3.1 grams of powder per liter of medium.

With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).

Media Formulation

Safety Information

• pictograms: GHS03
• signalword: Warning
• hcodes: H272
• pcodes: P210 - P220 - P280 - P370 + P378 - P501
• Hazard Classifications: Ox. Sol. 3
• Storage Class: 5.1B - Oxidizing hazardous materials
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable