F2677

Furin human

≥2,000 unit/mL, buffered aqueous solution, recombinant, expressed in baculovirus infected Sf9 cells

• Synonym(s): Dibasic-processing enzyme, Furin convertase, PACE, Paired basic amino acid residue-cleaving enzyme
• MDL number: MFCD03456906
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• form: buffered aqueous solution
• mol wt: 57 kDa
• concentration: ≥2,000 unit/mL
• UniProt accession no.: P09958
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... FURIN(5045)

Description

Application

Furin is capable of cleaving precursors of a wide variety of proteins, including growth factors, serum proteins, including proteases of the blood-clotting and complement systems, matrix metalloproteinases, receptors, viral-envelope glycoproteins, and bacterial exotoxins, typically at sites marked by the consensus sequence Arg-Xaa-(Lys/Arg)-Arg.

Biochem/physiol Actions

Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It has a molecular mass of 52.7 kDa. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells. Furin cleaves precursor proteins at their paired basic amino acid processing sites. Some substrates of furin include von Willebrand factor, transforming growth factor beta 1 precursor, pro-beta-secretase and proparathyroid hormone.

Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells.

Unit Definition

One unit is defined as the amount of enzyme required to cleave 25 μg of a MBP-FN-paramyosin-ΔSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 μl.

Physical form

Solution in 10 mM MES, pH 7.0 at 25 °C, 1 mM CaCl₂, 50% glycerol.

Preparation Note

Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable