B1064

Cibacron Blue 3G-A

Dye content ≥55 %, Powder

• Synonym(s): Cibacron Blue; 1-amino-4-[4-[[4-chloro-6-(2-sulfoanilino)-1,3,5-triazin-2-yl]amino]-3-sulfoanilino]-9,10-dioxoanthracene-2-sulfonic acid
• Empirical Formula (Hill Notation): C₂₉H₂₀ClN₇O₁₁S₃
• CAS Number: 84166-13-2
• Molecular Weight: 774.16
• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• product name: Cibacron Blue 3G-A, Dye content ≥55 %
• form: powder
• Quality Level: 200
• composition: Dye content, ≥55%
• color: dark blue
• solubility: H₂O: 10 mg/mL, blue
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Nc1c(cc(Nc2ccc(Nc3nc(Cl)nc(Nc4ccccc4S(O)(=O)=O)n3)c(c2)S(O)(=O)=O)c5C(=O)c6ccccc6C(=O)c15)S(O)(=O)=O
• InChI: 1S/C29H20ClN7O11S3/c30-27-35-28(33-16-7-3-4-8-19(16)49(40,41)42)37-29(36-27)34-17-10-9-13(11-20(17)50(43,44)45)32-18-12-21(51(46,47)48)24(31)23-22(18)25(38)14-5-1-2-6-15(14)26(23)39/h1-12,32H,31H2,(H,40,41,42)(H,43,44,45)(H,46,47,48)(H2,33,34,35,36,37)
• InChI key: YKCWQPZFAFZLBI-UHFFFAOYSA-N

Description

Application

When immobilized using an insoluble porous support matrix such as agarose, Cibacron Blue 3GA is used for affinity chromatography purification of enzymes. This affinity has been attributed to a structural similarity between the dye and the natural ligands for proteins with cofactor binding domains.

Biochem/physiol Actions

Cibacron Blue 3GA is an anionic anthraquinone dye. It acts as a P₂-purinoceptor antagonist and inhibits stimulus-evoked glutamate release by rat brain cortical tissue. Cibacron Blue 3GA inhibits OXA-1 and OXA-2 β-lactamases and was used to observe the binding of ligands to OXA-1 β-lactamase by monitoring tryptophan fluorescence.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable