A5043

Adenosine Deaminase from bovine spleen

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

• Synonym(s): ADA, adenosine aminohydrolase, Adenosine aminohydrolase
• CAS Number: 9026-93-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.5.4.4 (BRENDA, IUBMB)
• MDL number: MFCD00081287
• Specific activity: ≥130 units/mg protein
• Biological source: bovine spleen

Properties

• biological source: bovine spleen
• Quality Segment: 200
• type: Type X
• form: buffered aqueous glycerol solution
• specific activity: ≥130 units/mg protein
• mol wt: 32.5-33 kDa
• cation traces: NH₄⁺: ≤10 μg/mg protein
• UniProt accession no.: P56658
• foreign activity: alkaline phosphatase ≤0.01%, guanase ≤0.01%, nucleoside phosphorylase ≤0.1%
• storage temp.: 2-8°C
• Gene Information: cow ... ADA(280712)

Description

General description

Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays [1]. Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

Other Notes

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable