MAB331-C

Anti-SNAP-25 Antibody, clone SP14 (Ascites Free)

clone SP14, from mouse

• Synonym(s): Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP-25
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: SP14, monoclonal
• Application: EM, IHC, WB, activity assay
• Citations: 16

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: SP14, monoclonal
• species reactivity: monkey, bovine, mouse, rat, guinea pig, human
• technique(s): activity assay: suitable, electron microscopy: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_570824
• UniProt accession no.: P60880
• target post-translational modification: unmodified
• Gene Information: human ... SNAP25(6616)

Description

General description

Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25 kDa protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human. SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events. Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion. SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane. SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues.

~25 kDa observed

Immunogen

Epitope: N-terminal half

Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).

Application

Anti-SNAP-25 Antibody, clone SP14 (Ascites Free) is an antibody against SNAP-25 for use in Western Blotting, Immunohistochemistry (Paraffin), Electron Microscopy and Activity Assay.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Biochem/physiol Actions

Clone SP41 recognizes both SNAP-25 isoforms A & B. Clone SP41 is reactive toward endogenous or exogenously expressed SNAP-25 in COS cells, but not SNAP-25 expressed in E. coli, indicative of a posttranslational modification-dependent antigenicity.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNAP-25 in 10 µg of human brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: MAB331

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable