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Key Documents

MAB1612

Sigma-Aldrich

Anti-Cytokeratin Epithelial Antibody, clone AE1

clone AE1, Chemicon®, from mouse

Synonym(s):

Anti-CARD2, Anti-CK8, Anti-CYK8, Anti-K2C8, Anti-K8, Anti-KO

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AE1, monoclonal

species reactivity

mouse, chicken, human, rabbit, bovine, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KRT1(3848)
rat ... Krt19(360626)

Specificity

Recognizes most of the acidic (Type I) keratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. MAB1612 produces heterogeneous staining within a given epithelium; the detailed staining pattern varies depending on the differentiated and/or growth state of individual cells.

Immunogen

Human epidermal keratin

Application

Immunohistochemistry on unfixed frozen sections and formalin-fixed paraffin embedded tissues. Antigen retrieval is recommended. HEIR or protease treatment can be used.

Western Blotting: AE1 recognizes LMW cytokeratins from the acidic subfamily including: Clone AE1 recognises the 56.5, 50, 50′, 48 and 40kD human cytokeratins of the acidic subfamily.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Cytokeratins
This Anti-Cytokeratin Epithelial Antibody, clone AE1 is validated for use in IH(P) for the detection of Cytokeratin Epithelial.

Linkage

Replaces: 04-588

Physical form

Format: Purified
Liquid in 0.05M Borate Buffered Saline, 0.15M NaCl, 0.09% Sodium Azide, pH8.2.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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