614853

Topoisomerase II Inhibitor, BNS-22

The Topoisomerase II Inhibitor, BNS-22 controls the biological activity of Topoisomerase. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonym(s): Topoisomerase II Inhibitor, BNS-22, BNS-22
• Empirical Formula (Hill Notation): C₂₄H₂₅NO₅
• Molecular Weight: 407.46
• UNSPSC Code: 12352200
• NACRES: NA.54
• MDL number: MFCD22628799
• Assay: ≥99% (HPLC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Properties

• Quality Segment: 100
• assay: ≥99% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: white
• solubility: DMSO: 25 mg/mL, clear, colorless
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: N3(CCCc4c3cccc4)C(=O)c1c2c(c(cc1OC)OC)C(=CC(=O)O2)CCC
• InChI: 1S/C24H25NO5/c1-4-8-16-13-20(26)30-23-21(16)18(28-2)14-19(29-3)22(23)24(27)25-12-7-10-15-9-5-6-11-17(15)25/h5-6,9,11,13-14H,4,7-8,10,12H2,1-3H3
• InChI key: LRPUQCTZOSTSGL-UHFFFAOYSA-N

Description

General description

A potent, 2H-chromen-2-one small molecule inhibitor that is selective for TOP2 (IC₅₀ = 2.8 µM and 0.42 µM for TOP2α and TOP2β, respectively). Unlike TOP2 poisons such as etoposide, it does not lead to DNA damage associated with DNA Double-Strand Breaks, as evidenced by the lack of γ-H2AX accumulation but instead inhibits kinetoplast DNA decatenation in vitro. This compound affects cell cycle at the M phase and disturbs mitotic spindle formation in HeLa cells at 3-10 µM. It also exhibits significant anti-proliferative activities against several human cancer cell lines.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Kawatani, M., et al. 2011. Chem Biol.18, 743.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable