16-157
Protein A Agarose/Salmon Sperm DNA, 2.5 mL
for use in chromatin immunoprecipitations (ChIP assays)
Synonym(s):
ChIP agarose beads, ChIP agaraose A beads, ChIP assays
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| Size/SKU | Availability | Price |
|---|---|---|
2.5 mL | Check Cart for Availability | $463.00 |
About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Form:
liquid
Biological source:
Staphylococcus aureus
$463.00
Check Cart for Availability
biological source
Staphylococcus aureus
Quality Segment
form
liquid
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
suitability
suitable for immunoprecipitation
shipped in
wet ice
General description
Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
Application
Suitable for use in chromatin immunoprecipitation (ChIPs) assays
Physical form
10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.
Analysis Note
Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| biological source Staphylococcus aureus | biological source - | biological source - | biological source - |
| technique(s) ChIP: suitable | technique(s) ChIP: suitable | technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) - |
| form liquid | form liquid | form liquid | form slurry (Liquid) |
| suitability suitable for immunoprecipitation | suitability suitable for immunoprecipitation | suitability - | suitability - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level - |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
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