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05-793

Sigma-Aldrich

Anti-phospho-Estrogen Receptor alpha (Ser118) Antibody, clone NL44, rabbit monoclonal

clone NL44, Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

NL44, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pSer118)

Gene Information

human ... ESR1(2099)

Specificity

Predicted to cross-react with camel, mouse, pig and Syrian hamster based on sequence homology
phospho-estrogen receptor a (Ser118)

Immunogen

Peptide containing the sequence QLpSPF in which pS corresponds to phosphoserine at residue 118 of human estrogen receptor alpha

Application

Anti-phospho-Estrogen Receptor alpha (Ser118) Antibody, clone NL44 is a Rabbit Monoclonal Antibody for detection of ER alpha also known as Estrogen receptor alpha & has been tested in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

routinely evaluated by immunoblot on RIPA lysates from MCF-7 cells treated with estradiol and EGF, and recombinant estrogen receptor alpha phosphorylated in vitro with MAP Kinase 2/Erk2, active (Catalog #14-173)

Target description

~66kDa

Physical form

0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 100mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%
Format: Purified
Protein A purified

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dongsheng Chen et al.
Oncogene, 21(32), 4921-4931 (2002-07-16)
Estrogen receptor alpha (ERalpha) is a transcription factor that regulates expression of target genes in a ligand-dependent manner. Activation of gene expression is mediated by two transcription activation functions AF-1 and AF-2, which act in a promoter- and cell-specific manner.
Estradiol-induced phosphorylation of serine 118 in the estrogen receptor is independent of p42/p44 mitogen-activated protein kinase.
Joel, P B, et al.
The Journal of Biological Chemistry, 273, 13317-13323 (1998)
Yue Xue et al.
Molecular cancer research : MCR, 17(1), 238-249 (2018-09-13)
The presence of cancer stem cells (CSC), which possess the ability of self-renewal and cancer initiation, is correlated with poor prognosis and drug resistance of breast cancer patients. But the molecular regulatory networks for maintenance of CSC function still remain
Ze-Yi Zheng et al.
Cancer cell, 37(3), 387-402 (2020-03-07)
We report that neurofibromin, a tumor suppressor and Ras-GAP (GTPase-activating protein), is also an estrogen receptor-α (ER) transcriptional co-repressor through leucine/isoleucine-rich motifs that are functionally independent of GAP activity. GAP activity, in turn, does not affect ER binding. Consequently, neurofibromin

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