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  • Using the column wall itself as resistive heater for fast temperature gradients in liquid chromatography.

Using the column wall itself as resistive heater for fast temperature gradients in liquid chromatography.

Journal of chromatography. A (2015-10-20)
Ruben De Pauw, Matthias Pursch, Gert Desmet
ABSTRACT

A new system is proposed for applying fast temperature gradients in liquid chromatography. It consists of a 0.7 mm × 150 mm fused-silica column coated with a 50 μm Nickel-layer, which is connecting with a power source and a temperature control system to perform fast and reproducible temperature gradients using the column wall itself as a resistive heater. Applying a current of 4A and passive cooling results in a maximal heating and cooling rate of, respectively, 71 and -21 °C/min. Multi-segment temperature gradients were superimposed on mobile phase gradients to enhance the selectivity for three sets of mixtures (pharmaceutical compounds, a highly complex mixture and an insecticide sample). This resulted in a higher peak count or better selectivities for the various mixtures.

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