G005954
HPLC (Size Exclusion) Analysis of Proteins on Zenix®-C SEC-300, Effect of DTT and EDTA
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
mobile phase: (A) 150 mM sodium phosphate buffer, pH 7.0 (B) 150 mM sodium phosphate buffer, pH 7.0 with 2 mM DTT, 0.1 mM EDTA (C) 150 mM sodium phosphate buffer, pH 7.0 with 2 mM DTT, 0.1 mM EDTA
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μL
sample: proteins
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
Legal Information
analytical column
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