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RAB0556

Sigma-Aldrich

Human VWF / von Willebrand Factor ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

VWF ELISA Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 0.4 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VWF(7450)

General description

Human VWF/von Willebrand factor ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro assay for the quantitative measurement of a target protein in biological samples, such as serum, plasma, cell culture supernatants, urine and cell and tissue lysates.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human VWF/von Willebrand factor ELISA kit has been used for the quantification of endothelial marker-von Willebrand factor in bone marrow-derived mesenchymal stem cells (BMSC) and human umbilical vein endothelial cells (HUVEC).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J W R Sins et al.
Journal of thrombosis and haemostasis : JTH, 15(7), 1392-1402 (2017-05-01)
Essentials The role of von Willebrand Factor (VWF) in the pathophysiology of sickle cell disease is unclear. We assessed markers of VWF during admission for vaso-occlusive crisis (VOC) and steady state. VWF reactivity was higher during VOC and was associated
Tomasz Przygodzki et al.
Platelets, 29(5), 476-485 (2017-07-27)
Numerous in vitro experiments have confirmed that a dysfunctional endothelium is characterized by, inter alia, a higher affinity for binding of platelets and leukocytes. However, there is still no direct evidence for greater interaction between platelets and intact endothelium in
J Górka et al.
British journal of anaesthesia, 118(5), 713-719 (2017-05-10)
Myocardial injury after non-cardiac surgery (MINS), a complication with unclear pathogenesis, occurs within the first 30 days after surgery and worsens prognosis. Hypercoagulability induced by surgery might contribute to plaque rupture, with subsequent thrombosis and myocardial injury. This study assessed
Anne Sofie Gram et al.
Atherosclerosis, 265, 318-324 (2017-07-07)
Physical inactivity is linked to low-grade inflammation, endothelial dysfunction and cardiovascular disease. We aimed to determine effects of active commuting and leisure time exercise on markers of low-grade inflammation and endothelial function in overweight and obese women and men. We
Marko Stojanović et al.
Archives of physiology and biochemistry, 125(1), 44-55 (2018-02-13)
We have performed an in vitro study on isolated intact or denuded femoral artery (FA) of healthy, diabetic, and/or rats submitted to the FA occlusion. The aim was to determine the contribution of endothelium and endothelial dysfunction (ED) on serotonin-induced
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