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Key Documents

RAB0472

Sigma-Aldrich

Human TIMP-2 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

Metalloproteinase inhibitor 2 , TIMP-2, Tissue inhibitor of metalloproteinases 2

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1.6-1200 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TIMP2(7077)

General description

The Human TIMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIMP-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human TIMP2

Application

For research use only. Not for use in diagnostic procedures.
Human TIMP-2 ELISA Kit has been used to measure tissue inhibitor of metalloproteinase-2 (TIMP-2) level in blood samples from Atherosclerotic renal artery stenosis (ARAS) patients.

Biochem/physiol Actions

Tissue inhibitor of metalloproteinase-2 (TIMP-2) plays a vital role in inhibition of matrix metalloproteinases (MMPs) and functions as a key modulator of extracellular matrix deposition and degradation in various physiological processes. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. TIMP-2 mediates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22. TIMP-2 functions as a biomarker for cell cycle arrest.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Atherosclerotic renal artery stenosis is associated with elevated cell cycle arrest markers related to reduced renal blood flow and postcontrast hypoxia.
Saad A
Nephrology, Dialysis, and Transplantation, 31(11), 1855-1863 (2016)
TIMP-2 mediated inhibition of angiogenesis: an MMP-independent mechanism.
Seo DW
Cell, 114(2), 171-180 (2003)
Wei Wang et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 31(9), 1437-1443 (2016-02-26)
Atherosclerotic renal artery stenosis (ARAS) activates oxidative stress and chronic inflammatory injury. Contrast imaging and endovascular stenting pose potential hazards for acute kidney injury, particularly when superimposed upon reduced kidney perfusion. We measured sequential early and long-term changes in circulating
Tissue inhibitors of metalloproteinases: structure, regulation and biological functions.
Gomez DE
European Journal of Cell Biology, 74(2), 111-122 (1997)
Changes in inflammatory biomarkers after renal revascularization in atherosclerotic renal artery stenosis.
Wang W
Nephrology, Dialysis, and Transplantation, 31(9), 1437-1443 (2016)
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