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Key Documents

MAK125

Sigma-Aldrich

BCP Albumin Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Bromocresol Purple Albumin Assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 250 colorimetric tests

detection method

colorimetric

relevant disease(s)

genitourinary disorders; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... ALB(213)
mouse ... ALB(11657)
rat ... ALB(24186)

General description

Albumin is the most abundant plasma protein in humans. It accounts for ~60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure and binding of key substances such as long-chain fatty acids, bile acids, bilirubin, hematin, calcium, and magnesium. It has antioxidant and anticoagulant effects, acts as a carrier for nutritional factors and drugs, and is an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition, and protein-losing enteropathies. High levels are associated with dehydration.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

The BCP Albumin Assay Kit is designed to measure albumin directly without any pretreatment of samples, such as serum, plasma, urine, and biological preparations. The optimized formulation substantially reduces interference by other substances (lipids/other proteins) in the raw samples. It may also be used to measure effects of drugs and other compounds on albumin metabolism.

Principle

The procedure involves addition of a single working reagent and a 5 minute incubation. The optimized formulation has greatly enhanced reagent and signal stability. The kit utilizes bromocresol purple, which forms a colored complex specifically with albumin. The intensity of the color, measured at 610 nm, is directly proportional to the albumin concentration in the sample.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alexander H Penn et al.
Shock (Augusta, Ga.), 53(2), 179-188 (2019-03-05)
Military prehospital care for hemorrhage is often characterized by use of tourniquets (TQ) and permissive hypotensive resuscitation (PHR) with crystalloids or colloids, but these treatments have not been previously combined in an animal model. Although albumin resuscitation solutions have been
Doaa I Mohamed et al.
Pharmaceuticals (Basel, Switzerland), 15(7) (2022-07-28)
Acute heart failure (AHF) is one of the most common diseases in old age that can lead to mortality. Systemic hypoperfusion is associated with hepatic ischemia-reperfusion injury, which may be irreversible. Ischemic hepatitis due to AHF has been linked to

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