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Key Documents

L2025

Sigma-Aldrich

Luria Agar Base, Miller

Tryptone, yeast extract, sodium chloride and agar

Synonym(s):

LA, modified, Luria-Bertani media, modified, Lysogeny broth, modified

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About This Item

UNSPSC Code:
41106200
eCl@ss:
42040102
NACRES:
NA.72

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

General description

Luria Bertani (LB) medium is one of the complex media that is widely used to grow Escherichia coli in small-scale cultures.

Application

Luria Agar Base, Miller has been used as a growth medium during plasmid amplification. It has also been used as a culture medium for strains of Penicillium sp. laika.
Luria broth/agar (LA), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) yeast extract (5g/L) and agar (15g/L) used to culture members of the Enterobacteriaceae family and for coliphage plaque assays.

Components

Contains: Tryptone, yeast extract, sodium chloride and agar.

Reconstitution

Recommended use at 30.5 g per liter.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Chujin Ruan et al.
Current biology : CB, 32(24), 5285-5294 (2022-12-02)
The amount of bacterial diversity present on many surfaces is enormous; however, how these levels of diversity persist in the face of the purifying processes that occur as bacterial communities expand across space (referred to here as range expansion) remains
Daria Julkowska et al.
Journal of bacteriology, 187(1), 65-76 (2004-12-17)
The natural wild-type Bacillus subtilis strain 3610 swarms rapidly on the synthetic B medium in symmetrical concentric waves of branched dendritic patterns. In a comparison of the behavior of the laboratory strain 168 (trp) on different media with that of
Anthony S Danko et al.
Applied and environmental microbiology, 70(10), 6092-6097 (2004-10-07)
Pseudomonas putida strain AJ and Ochrobactrum strain TD were isolated from hazardous waste sites based on their ability to use vinyl chloride (VC) as the sole source of carbon and energy under aerobic conditions. Strains AJ and TD also use
Xiaoyun Qiu et al.
Applied and environmental microbiology, 70(11), 6435-6443 (2004-11-06)
We systematically investigated the physiological response as well as DNA damage repair and damage tolerance in Shewanella oneidensis MR-1 following UVC, UVB, UVA, and solar light exposure. MR-1 showed the highest UVC sensitivity among Shewanella strains examined, with D37 and
Mario Losen et al.
Biotechnology progress, 20(4), 1062-1068 (2004-08-07)
Shake-flask cultures are widely used for screening of high producing strains. To select suitable strains for production scale, cultivation parameters should be applied that provide optimal growth conditions. A novel method of measuring respiratory activity in shake-flask cultures was employed

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