Skip to Content
Merck
All Photos(1)

Documents

F9259

Sigma-Aldrich

Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Mouse IgM antibody

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:128

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Mouse IgM (μ-chain specific)-FITC antibody is specific for mouse IgM with mu chain. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM. Goat anti-mouse IgM is then conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Specificity

Binds mouse IgM only; does not bind other mouse Igs.

Immunogen

Purified mouse IgM

Application

Anti-Mouse IgM (μ-chain specific)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a minimum working antibody dilution of 1:128. It was also used for immunohistochemistry of rat heart tissue sections at a working dilution of 1:50. A dilution of 1:100 was used to label human ovarian-adenocarcinoma cells for immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

F Carreiras et al.
International journal of cancer, 63(4), 530-536 (1995-11-15)
Accumulating evidence suggests that integrins, which participate in many complex cellular processes, are important for tumor progression and metastasis. In order to understand the role of these cell-surface receptors and of their ligands in the biological behavior of ovarian tumor
Bone marrow stromal cells improve cardiac performance in healed infarcted rat hearts
Olivares EL et al
American Journal of Physiology. Heart and Circulatory Physiology, 287, H646-H670 (2004)
Chenjie Zhou et al.
Hematology (Amsterdam, Netherlands), 22(2), 119-127 (2016-07-16)
Monoclonal anti-human blood group A (51A8) and B (63B6) antibody reagents were prepared using the serum-free technique. The aims of this research were to characterize the serum-free reagents and prove their reliabilities in routine use. Experiments including antigen-antibody agglutination testing
C R Thornton et al.
Phytopathology, 87(1), 123-131 (1997-01-01)
ABSTRACT A murine monoclonal antibody (MAb) of immunoglobulin class M (IgM) was raised against surface antigens from Gaeumannomyces graminis var. tritici and, by enzyme-linked immunosorbent assay, recognized isolates of G. graminis var. tritici, G. graminis var. avenae and G. graminis
Joanna R Morris et al.
Human molecular genetics, 13(8), 807-817 (2004-02-21)
The N-terminus of the BRCA1 protein bears a RING finger domain that functions as an E3 ubiquitin ligase in vitro where it is able to catalyse the synthesis of monoubiquitin and polyubiquitin targeted proteins. This activity is greatly increased when

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service