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Key Documents

SW 403

87071008, human colon, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

SW 403, 87071008

biological source

human colon

growth mode

Adherent

karyotype

2n = 46, triploid

morphology

Epithelial

products

Carcinoembryonic antigen (CEA)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.

Application

Tumourigenicity studies

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37°C. Cells do not reach confluency but will grow in small islands.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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