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MABN1833

Sigma-Aldrich

Anti-nAChR α4 Subunit Antibody, clone 369

clone 369, from mouse

Synonym(s):

Neuronal acetylcholine receptor subunit alpha-4, alpha4 nAChR subunit, Cholinergic receptor, nicotinic, alpha polypeptide 4, nAChR alpha4 subunit, Neuronal nicotinic acetylcholine receptor alpha-4 subunit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

369, monoclonal

species reactivity

monkey, human, rabbit

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1κ

NCBI accession no.

NP_000735

UniProt accession no.

P43681

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CHRNA4(1137)

General description

Neuronal acetylcholine receptor subunit alpha-4 (UniProt P43681; also known as alpha4 nAChR subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, nAChR alpha4 subunit, Neuronal nicotinic acetylcholine receptor alpha-4 subunit) is encoded by the CHRNA4 (also known as NACRA4, NACHRA4) gene (Gene ID 1137) in human. The nicotinic acetylcholine (ACh) receptors (nAChRs) are activated by acetylcholine (ACh) and nicotinic agonists, such as cytisine, 1,1-dimethyl-4-phenylpiperazinium and carbachol. The nAChRs are ligand-gated pentameric cation channels. Currently, 12 neuronal and 5 muscle nAChR subunits have been identified. The nAChRs can exist as homopentamers of five identical alpha subunits (α7, α8, or α9), alpha heteropentamers composed of more than one type of alpha subunits (α7-α10), or as alpha/beta heteropentamers consisting of various combinations of alpha (α2-α6) and beta (β2-β4) subunits. The heteromeric alpha/beta types display high ligand affinity, but are insensitive to α-bungarotoxin (αBgt), while the alpha-only homomeric and heteromeric pentamers show lower agonist affinity, but high αBgt sensitivity. The α4β2 (two α4 and three β2 subunits) and α7 (five α7 subunits) nAChRs are the predominant nAChRs in the central nervous system (CNS) among mammalian species. The alpha-4 subunit is initially produced with a signal peptide (a.a. 1-28), the removal of which yields the mature 627-amino acid membrane protein with a large extracellular domain (a.a. 29-242), four transmembrane segments (a.a. 243-267, 275-293, 309-330, 601-619), and a large cytoplasmic loop (a.a. 331-600) between the third and fourth transmembrane domains.

Immunogen

Recombinant human nAChR α4 subunit.

Application

Anti-nAChR α4 Subunit Antibody, clone 369 is an antibody against nAChR α4 Subunit for use in Immunohistochemistry (Paraffin), Immunofluorescence.
Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected nAChR α4 Subunit in human small intestine tissue.
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in monkey frozen retina sections by fluorescence immunohistochemistry (Liu, J., et al. (2009). Invest. Ophthalmol. Vis .Sci. 50(3):1408-1415).
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in various areas of the rabbit brainstem by fluorescence immunohistochemical staining of free-floating sections (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Immunofluorescence Analysis: A represenative lot detected an increase of nAChR α4-positive neurons in the A1, A2 and A6 brainstem noradrenergic areas of female rabbits 1 hr after mating by fluorescence immunohistochemical staining of free-floating sections. The number of nAChR α4-positive neurons subsided to basal level 2 hrs after mating (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Quality

Evaluated by Immunohistochemistry in human cerebellum tissue.

Immunohistochemistry Analysis: An 1:50 dilution of this antibody detected nAChR α4 Subunit in human cerebellum tissue.

Target description

67.16 kDa calculated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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