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  • Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans.

Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans.

Archives of biochemistry and biophysics (2009-12-01)
Yong-Hak Kim, Cheolju Lee, Hayoung Go, Kyoko Konishi, Kangseok Lee, Peter C K Lau, Myeong-Hee Yu
ABSTRACT

We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was determined by optical absorbance spectroscopy with an extinction coefficient (E(550-535)) of 19.7+/-6.3 mM(-1) cm(-1) and reduction potential of + 261 mV. When purified CeCyt was added into the mitochondria, the specific activity of CeCyt reached 440 +/- 122 micromol min(-1) mg(-1) protein. The inhibition of MG reduction by stigmatellin, but not by antimycin A, indicated a possible linkage of CeCyt activity to the Qo site of the bc1 complex. The RT-PCR results showed tight regulation of the cecyt gene expression by reactive oxygen species. We suggest that CeCyt acts as a protein reductant for MG under oxidative stress in a stationary or secondary growth stage of this fungus.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Brilliant Blue R, pure
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Brilliant Blue R Staining Solution, suitable for (for immunoelectrophoresis protein staining)
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Brilliant Blue R Concentrate, suitable for SDS-PAGE, methanol solution
Sigma-Aldrich
Brilliant Blue R, Dye content ~50 %, Technical grade
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Brilliant Blue R, 250, for microscopy