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  • Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine.

Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine.

STAR protocols (2020-10-29)
Bert Dhondt, Nicolaas Lumen, Olivier De Wever, An Hendrix
ABSTRACT

The multidimensional cargo of extracellular vesicles (EV) released in urine is a reflection of the pathophysiological processes occurring within their cells and tissues of origin in the urogenital system. Here, we describe a step-by-step protocol for density-based separation of urinary EV with high specificity and repeatability. The implementation of integrative omics allows the study of the molecular complexity of highly purified urinary EV, supporting the identification of EV-specific functions and biomarkers. For complete details on the use and execution of this protocol, please refer to Dhondt et al. (2020).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-UPK1B antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Sucrose, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Anti-PMP70 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution