Silica nanoparticles with Tb(III)-centered luminescence decorated by Ag0 as efficient cellular contrast agent with anticancer effect.

Silica nanoparticles with Tb(III)-centered luminescence decorated by Ag0 as efficient cellular contrast agent with anticancer effect.

Journal of inorganic biochemistry (2018-02-28)

Svetlana V Fedorenko, Svetlana L Grechkina, Alsu R Mukhametshina, Anastasiya O Solovieva, Tatiana N Pozmogova, Svetlana M Miroshnichenko, Alexander Y Alekseev, Michael A Shestopalov, Kirill V Kholin, Irek R Nizameev, Asiya R Mustafina

PMID29486416

ABSTRACT

The present work introduces composite luminescent nanoparticles (Ag0-Tb3+-SNs), where ultra-small nanosilver (4 ± 2 nm) is deposited onto amino-modified silica nanoparticles (35±6 nm) doped by green luminescent Tb(III) complexes. Ag0-Tb3+-SNs are able to image cancer (Hep-2) cells in confocal microscopy measurements due to efficient cell internalization, which is confirmed by TEM images of the Hep-2 cells exposed by Ag0-Tb3+-SNs. Comparative analysis of the cytotoxicity of normal fibroblasts (DK-4) and cancer cells (Hep-2) incubated with various concentrations of Ag0-Tb3+-SNs revealed the concentration range where the toxic effect on the cancer cells is significant, while it is insignificant towards the nonmalignant fibroblasts cells. The obtained results reveal Ag0-Tb3+-SNs as good cellular contrast agent able to induce the cancer cells death, which makes them promising theranostic in cancer diagnostics and therapy.

MATERIALS

Product Number

Brand

Product Description

217212

Sigma-Aldrich

Terbium(III) nitrate hexahydrate, 99.999% trace metals basis

X100

Sigma-Aldrich

Triton^™ X-100, laboratory grade