WH0004149M1

Monoclonal Anti-MAX antibody produced in mouse

clone 4E10-1A9, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-MGC10775, Anti-MGC11225, Anti-MGC18164, Anti-MGC34679, Anti-MGC36767, Anti-MYC associated factor X, Anti-orf1
• MDL number: MFCD01633623
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4E10-1A9, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): immunofluorescence: suitable; indirect ELISA: suitable; proximity ligation assay: suitable; western blot: 1-5 μg/mL
• isotype: IgG2aκ
• GenBank accession no.: BC003525
• UniProt accession no.: P61244
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAX(4149)

Description

General description

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown. (provided by RefSeq)

Immunogen

MAX (AAH03525, 1 a.a. ~ 151 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

Biochem/physiol Actions

Myc-associated factor X (MAX) interacts with N-, L-, and c-Myc proteins both in vitro and in vivo. MAX plays a vital role in the regulation of the MYC/MAX/ MAX dimerization protein (MXD) network of basic helix-loop-helix leucine zipper (bHLHZip) transcription factors implicated in cell proliferation, differentiation, and apoptosis. Mutation of the gene leads to pheochromocytomas and paragangliomas (PPGLs). Heterodimer complex of c-Myc and Max proteins stimulates the transcription at target promoters by binding hexameric DNA consensus sequences “CANNTG” (E-boxes). Apart from this, Max hinders MYC-dependent cell transformation by transcriptional repression of the same E-boxes by associating with various repressors such as MXD, MAX network transcriptional repressor (MNT), and MAX gene-associated protein (MGA). Gene transcription activated by MYC–MAX heterodimers can be repressed by Max homodimers by decreasing the level of free MAX monomers and by binding to E-box DNA sequences.

Physical form

Solution in phosphate buffered saline, pH 7.4

Legal Information

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Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves