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AB2251-I

Sigma-Aldrich

Anti-VGluT2 Antibody

serum, from guinea pig

Synonym(s):

Vesicular glutamate transporter 2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporte, Solute carrier family 17 member 6, VGluT2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

guinea pig

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

General description

Vesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporte, Solute carrier family 17 member 6, VGluT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID 84487) in rat species. Vesicular glutamate transporters (VGluTs) are proton-dependent carriers responsible for the upload of the major excitatory neurotransmitter glutamate into synaptic vesicles within presynaptic terminals to allow later regulated release at the synaptic cleft. Studies conducted in mice show that VGluT1 and VGluT2 are essential for vital functions, while Vglut3-knockout mice have a normal life expectancy despite being deaf and developing anxiety and discrete basal locomotor phenotypes. VgluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VgluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. In contrast, VgluT3 is found in discrete population of non-glutamatergic neurons using other transmitters than glutamate, such as cholinergic interneurons in the dorsal and ventral striatum, subpopulations of GABAergic basket cells in the cortex and hippocampus, and serotoninergic neurons, where it is involved in more subtle modulation of local transmission. Rat VGluT2 is a 12-transmembrane (a.a. 72-92, 126-146, 149-169, 178-198, 217-237, 245-265, 311-331, 350-370, 387-407, 410-430, 444-464, 478-498) protein with 7 cytoplasmic and 6 vacuolar domains, having both its N- and C-terminal tails (a.a. 1-71 and 499-582) exposed at the cytoplasmic side.

Specificity

This polyclonal antibody targets VGluT2 C-terminal end sequence.

Immunogen

Epitope: C-terminus.
KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat VGluT2.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected VGluT2 in mouse and rat brain tissue sections.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
This Anti-VGluT2 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of VGluT2.

Quality

Evaluated by Western Blotting in rat brain membrane extract.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected VGluT2 in 10 µg of rat brain membrane extract.

Target description

~56 kDa observed. 64.58/64.56 kDa (rat/mouse) calculated. The smaller than predicted band size is consistent with the reported apparent molecular weight (Zhang, S., et al. (2015). Nat. Neurosci. 18(3):386-392; Moechars, D., et al. (2006). J. Neurosci. 26(46):12055-12066). Uncharacterized band(s) may appear in some lysates.

Physical form

Guinea pig polyclonal antibody serum with 0.05% sodium azide.
Unpurified.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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