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Key Documents

14-659

Sigma-Aldrich

LIM Kinase 1 Protein, inactive, 50 g

Unactive, N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 41.8 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... LIMK1(3984)

General description

N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638.
Product Source: Expressed by baculovirus in Sf21 cells.

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Protein Target: LIMK1
Target Sub-Family: TKL

Quality

routinely evaluated by phosphorylation of Cofilin 1 (Catalog # 12-556).

Physical form

Ni2+/NTA-agarose

Storage and Stability

6 months at -70°C from date of shipment

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Franco Spelta et al.
Frontiers in medicine, 6, 78-78 (2019-05-07)
Heparin is a complex mixture of heterogeneous sulfated polysaccharidic chains. Its physico-chemical characterization is based on the contribution of several methods, but advantages of the use of complementary techniques have not been fully investigated yet. Strong-Anion-Exchange HPLC after enzymatic digestion

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