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  • Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark.

Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark.

Nature chemical biology (2014-04-01)
Lunzhi Dai, Chao Peng, Emilie Montellier, Zhike Lu, Yue Chen, Haruhiko Ishii, Alexandra Debernardi, Thierry Buchou, Sophie Rousseaux, Fulai Jin, Benjamin R Sabari, Zhiyou Deng, C David Allis, Bing Ren, Saadi Khochbin, Yingming Zhao
ABSTRACT

We report the identification of a new type of histone mark, lysine 2-hydroxyisobutyrylation (Khib), and identify the mark at 63 human and mouse histone Khib sites, including 27 unique lysine sites that are not known to be modified by lysine acetylation (Kac) and lysine crotonylation (Kcr). This histone mark was initially identified by MS and then validated by chemical and biochemical methods. Histone Khib shows distinct genomic distributions from histone Kac or histone Kcr during male germ cell differentiation. Using chromatin immunoprecipitation sequencing, gene expression analysis and immunodetection, we show that in male germ cells, H4K8hib is associated with active gene transcription in meiotic and post-meiotic cells. In addition, H4K8ac-associated genes are included in and constitute only a subfraction of H4K8hib-labeled genes. The histone Khib mark is conserved and widely distributed, has high stoichiometry and induces a large structural change. These findings suggest its critical role on the regulation of chromatin functions.

MATERIALS
Product Number
Brand
Product Description

Lysine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
L-Lysine, crystallized, ≥98.0% (NT)
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α-Hydroxyisobutyric acid, 99%
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α-Hydroxyisobutyric acid, 98%
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L-Lysine acetate salt, ≥98% (HPLC)
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L-Lysine, ≥98% (TLC)
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L-Lysine, analytical standard
Lysine acetate, European Pharmacopoeia (EP) Reference Standard
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L-Lysine monohydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
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L-Lysine monohydrochloride, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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L-Lysine Acetate, Pharmaceutical Secondary Standard; Certified Reference Material
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L-Lysine monohydrochloride, reagent grade, ≥98% (HPLC)
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L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
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L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)