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  • [Change of endogenous hormone around sprout tumble of Pinellia ternata under high temperature stress].

[Change of endogenous hormone around sprout tumble of Pinellia ternata under high temperature stress].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2008-03-12)
Jian-Ping Xue, Ai-Min Zhang, Jian Yang, Li Chang, Yue-Qin Huang
ABSTRACT

To study the change of endogenous hormone (ABA, IAA, JA, GA3, ZR) in the leaves, petioles, tubers of Pinellia ternate around sprout tumble. It also provided some valuable information to prevent sprout tumble and increase production. Tubers of P. ternata were cultured firstly at (23 +/- 1) degree C for certain days, and then they were coerced under (30 +/- 1 ) degree C stress in the same artificial climate boxes. The endogenous hormones in leaves, petioles and tubers during different stages of high temperature stress were determined with Enzyme-linked Immunosorbent Assays (ELISA). After under high temperature stress, ABA content in leaves, petioles and tubers increased obviously. Similarly, JA content rose all in the leaves, petioles and tubers. But in the same conditions IAA content declined significantly in the leaves and petioles. In the tubers, IAA content also decreased, but not quickly. With the extension of high temperature coercion, the leaves, petioles, tubers, ZR content were gradually falling off. In the leaves of GA3 content rose markedly at the third day, fell down at the sixth day, but remained higher than before treatment. With the extension of the processing time, GA3 content fell off in the petioles and tubers. ABA, JA, ZT and GA3 played an important role in controlling sprout tumble of P. ternata.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
trans-Zeatin-riboside, ~95%
Sigma-Aldrich
trans-Zeatin-riboside, suitable for plant cell culture, BioReagent, ~95%