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  • Flow cytometric assessment of ethylene glycol monoethyl ether on spermatogenesis in rats.

Flow cytometric assessment of ethylene glycol monoethyl ether on spermatogenesis in rats.

The Journal of veterinary medical science (2003-03-26)
Chang Yong Yoon, Choong Man Hong, Yong-Yeon Cho, Yong Hyun Chung, Hong Ki Min, Young Won Yun, Beom Jun Lee, Ki Hwa Yang, Yong Soon Lee, Cheol Kyu Kim
ABSTRACT

The effects of ethylene glycol monoethyl ether (EGEE) on testicular cell populations in rats were investigated by a flow cytometric method. Rats were administered by gavage with EGEE at the various doses of 0 (saline alone), 100, 200, 400, and 800 mg/kg body weight/day for 4 weeks. The treatment of EGEE caused decreases in the weight of testis and epididymis and in the number of testicular cells. Histopathologically, exfoliation of germ cells into the tubular lumen was observed at the doses of above 200 mg/kg. The treatment of EGEE at the dose of 400 mg/kg caused moderate testicular degeneration. A significant depletion of haploid cells and a disproportionate ratio of diploid and tetraploid cells were observed as determined by flow cytometric analysis. These results indicate that the toxic effect of EGEE on the male reproductive system may be strongly associated with the disproportion of testicular germ cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Ethoxyethanol, spectrophotometric grade, ≥99%
Supelco
2-Ethoxyethanol, analytical standard
Sigma-Aldrich
2-Ethoxyethanol, ReagentPlus®, 99%