03978

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

• Synonym(s): Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue
• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S
• CAS Number: 61-73-4
• Molecular Weight: 319.85
• Beilstein: 3641570
• MDL number: MFCD00012111
• UNSPSC Code: 12171500
• PubChem Substance ID: 329747853
• NACRES: NA.47

Properties

• grade: for microscopy
• form: liquid
• quality: concentrated, aqueous solution
• shelf life: ~3 yr
• concentration: 1.5% (1.5 g/ 100 mL)
• density: 1.0 g/mL at 20 °C
• εmax: 9.4 at 656-666 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

General description

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves