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N5514

Sigma-Aldrich

Nitro Blue Tetrazolium

tablet

Synonym(s):

Nitrotetrazolium Blue Chloride

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

tablet

Quality Level

color

yellow

pH

4.5-5.5

solubility

soluble, clear, yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

General description

Nitro Blue Tetrazolium is a tetrazole compound with two tetrazole rings.

Application

Nitro Blue Tetrazolium has been used:
  • to measure superoxide radicals
  • in lactate dehydrogenase quantification assay
  • for blot development in western blotting analysis
  • in NADPH-d histochemistry
  • as a reagent to analyse the properties of murine intestinal mucins by electrophoresis and histology
  • in immunohistochemistry

Quantity

Contains 10 mg substrate per tablet.

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Nitrotetrazolium blue chloride as a novel corrosion inhibitor of steel in sulfuric acid solution
Deng S, et al
Corrosion Science, 52(11), 3840-3846 (2010)
Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
Wang R and Hasnain SZ
PLoS Pathogens (2017)
Salmonella Serovars and Vaccination Effect on the Immune Responses of Male and Female Layers
Chen Kl, et al.
The journal of poultry science, 53(4), 318-328 (2016)
Night light-adaptation strategies for photosynthetic apparatus in yellow-poplar (Liriodendron tulipifera L.) exposed to artificial night lighting
Kwak MJ, et al.
Forests, 9(2), 74-74 (2018)
Transient expression of a bovine leukemia virus envelope glycoprotein in plants by a recombinant TBSV vector
Zhumabek AT, et al.
Journal of Virological Methods, 255, 1-7 (2018)

Articles

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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