HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)
Materials
standard
Product No.
Description
Pricing
Cytochrome c from bovine heart
≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell cultureRibonuclease A from bovine pancreas
Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg proteinRibonuclease A from bovine pancreas
for molecular biology, ≥70 Kunitz units/mg protein, lyophilizedRibonuclease A from bovine pancreas
Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg proteinCytochrome c from equine heart
BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell cultureCONDITIONS
column
Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase
[A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear)
flow rate
0.5 mL/min
injection
10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
detector
UV, 280 nm
Description
Analysis Note
The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany