HPLC Analysis of Alanine Enantiomers on Astec^® CHIROBIOTIC^® T (No Mobile Phase Modifier)

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] acetonitrile; (50:50, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

1 mg/mL in methanol