A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.

A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.

Soft matter (2014-11-20)

B Frohm, J E DeNizio, D S M Lee, L Gentile, U Olsson, J Malm, K S Akerfeldt, S Linse

ABSTRACT

The peptide GSFSIQYTYHV derived from human semenogelin I forms a transparent hydrogel through spontaneous self-assembly in water at neutral pH. Linear rheology measurements demonstrate that the gel shows a dominating elastic response over a large frequency interval. CD, fluorescence and FTIR spectroscopy and cryo-TEM studies imply long fibrillar aggregates of extended β-sheet. Dynamic light scattering data indicate that the fibril lengths are of the order of micrometers. Time-dependent thioflavin T fluorescence shows that fibril formation by GSFSIQYTYHV is a nucleated reaction. The peptide may serve as basis for development of smart biomaterials of low immunogenicity suitable for biomedical applications, including drug delivery and wound healing.

MATERIALS

Product Number

Brand

Product Description

43617

Supelco

Sodium hydroxide concentrate, 0.1 M NaOH in water (0.1N), Eluent concentrate for IC

75746

Sigma-Aldrich

Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets

71687

Sigma-Aldrich

Sodium hydroxide, BioUltra, for luminescence, ≥98.0% (T), pellets

72068

Sigma-Aldrich

Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H₂O

71717

Sigma-Aldrich

Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

72064

Supelco

Sodium hydroxide solution, 49-51% in water, eluent for IC

227056

Sigma-Aldrich

N,N-Dimethylformamide, anhydrous, 99.8%

71726

Supelco

Sodium dodecyl sulfate, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

62862

Sigma-Aldrich

Sodium dodecyl sulfate, ≥90% ((Assay))

72438

Supelco

N,N-Dimethylformamide, analytical standard

71725

Sigma-Aldrich

Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

437573

Sigma-Aldrich

N,N-Dimethylformamide, ACS reagent, ≥99.8%

436143

Sigma-Aldrich

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

71729

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

L5750

Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

L6026

Sigma-Aldrich

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

74255

Supelco

Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

S8045

Sigma-Aldrich

Sodium hydroxide, BioXtra, ≥98% (acidimetric), pellets (anhydrous)

S2770

Sigma-Aldrich

Sodium hydroxide solution, 1.0 N, BioReagent, suitable for cell culture

M2128

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, 98%

L4509

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

T28002

Sigma-Aldrich

Thioanisole, ReagentPlus^®, ≥99%

M5655

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

D4551

Sigma-Aldrich

N,N-Dimethylformamide, for molecular biology, ≥99%

270547

Sigma-Aldrich

N,N-Dimethylformamide, suitable for HPLC, ≥99.9%

282979

Sigma-Aldrich

3-Ethyl-2,4-pentanedione, mixture of tautomers, 98%

L3771

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

901915

Sigma-Aldrich

Sodium hydroxide, anhydrous, free-flowing, Redi-Dri^™, reagent grade, ≥98%, pellets

V800383

Sigma-Aldrich

Sodium hydroxide, AR, pellets, ≥98.5%

30620

Sigma-Aldrich

Sodium hydroxide, puriss. p.a., ACS reagent, reag. Ph. Eur., K ≤0.02%, ≥98%, pellets