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Quantification analysis of yeast-displayed lipase.

Analytical biochemistry (2014-01-11)
Zi Jin, Kun Zhang, Li Zhang, Sui-Ping Zheng, Shuang-Yan Han, Ying Lin
ABSTRACT

We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as "g/g cell" or "molecules/cell". The results obtained correlated well with those obtained by existing methods. Therefore, this method is credible and will provide a powerful tool to promote research of lipase yeast surface display.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methyl 4-methylumbelliferyl hexylphosphonate, ≥95.0% (HPLC)
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