Skip to Content
Merck
  • Probing a 2-aminobenzimidazole library for binding to RNA internal loops via two-dimensional combinatorial screening.

Probing a 2-aminobenzimidazole library for binding to RNA internal loops via two-dimensional combinatorial screening.

ACS chemical biology (2012-09-11)
Sai Pradeep Velagapudi, Alexei Pushechnikov, Lucas P Labuda, Jonathan M French, Matthew D Disney
ABSTRACT

There are many potential RNA drug targets in bacterial, viral, and human transcriptomes. However, there are few small molecules that modulate RNA function. This is due, in part, to a lack of fundamental understanding about RNA-ligand interactions including the types of small molecules that bind to RNA structural elements and the RNA structural elements that bind to small molecules. In an effort to better understand RNA-ligand interactions, we diversified the 2-aminobenzimidazole core (2AB) and probed the resulting library for binding to a library of RNA internal loops. We chose the 2AB core for these studies because it is a privileged scaffold for binding RNA based on previous reports. These studies identified that N-methyl pyrrolidine, imidazole, and propylamine diversity elements at the R1 position increase binding to internal loops; variability at the R2 position is well tolerated. The preferred RNA loop space was also determined for five ligands using a statistical approach and identified trends that lead to selective recognition.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Aminobenzimidazole, 97%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 mg
Please contact Customer Service for Availability
HUF 161,000.00
Supelco
2-Aminobenzimidazole, PESTANAL®, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 mg
Please contact Customer Service for Availability
HUF 161,000.00