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Key Documents

B9804

Sigma-Aldrich

Anti-Bcl-2 antibody, Mouse monoclonal

clone 10C4, purified from hybridoma cell culture

Synonym(s):

Anti-BCL-2

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10C4, monoclonal

form

buffered aqueous solution

mol wt

antigen 26 kDa

species reactivity

rat, mouse

concentration

~2 mg/mL

technique(s)

microarray: suitable
western blot: 2-10 μg/mL using rat osteosarcoma ROS cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Bcl2(12043)
rat ... Bcl2(24224)

Specificity

The epitope recognized by the antibody resides within amino acids 61-76 of the mouse Bcl-2 protein.

Immunogen

synthetic peptide corresponding to amino acids 61-76 of the mouse Bcl-2 sequence, conjugated to KLH.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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