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05-1076

Sigma-Aldrich

Anti-c-Jun Antibody, clone 6E4.4

clone 6E4.4, from mouse

Synonym(s):

JNK1 alpha protein kinase, JNK1 beta protein kinase, JUN N-terminal kinase, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated pro

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6E4.4, monoclonal

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μg

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_002219.1

UniProt accession no.

P45983

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... JUN(3725)
mouse ... Jun(16476)
rat ... Jun(24516)

General description

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Specificity

This antibody detects c-Jun, regardless of phosphorylation state.

Immunogen

GST-tagged recombinant protein corresponding to c-Jun.

Application

Anti-c-Jun Antibody, clone 6E4.4 is a Mouse Monoclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique).

Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.

Target description

~36-43 kDa
Multiple bands may be visible, indicating various phosphorylation states.

Linkage

Replaces: 04-210

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable at 2-8°C for 1 year from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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