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P9740

Sigma-Aldrich

PHOS-Select™ Iron Affinity Gel

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

form

suspension

shelf life

2 yr at −20‑0 °C

pH

~5

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

storage temp.

−20°C

General description

The PHOS-Select™ iron affinity gel is a novel iron [Fe(III)] chelate matrix. This matrix provides high capacity affinity binding of molecules containing phosphate groups.

Application

Phos-Select™ products facilitate a number of applications including:
  • affinity enrichment of phosphopeptides obtained from protein tryptic digests
  • direct transfer of phosphocompounds for analysis (HPLC or mass spectrometry)
  • enrichment of phosphopeptides by immobilized metal affinity chromatography (IMAC)

Other Notes

Phos-Select™ Iron Affinity Gel is a novel iron [Fe(III)] chelate matrix based on Sigma′s NTA analog chelate ligand. The matrix provides high capacity affinity binding of molecules containing phosphate groups.

Physical form

aqueous glycerol suspension

Legal Information

PHOS-Select is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Quantitative Phosphoproteomics of the ATM and ATR dependent DNA damage response in Arabidopsis thaliana
Roitinger E, et al.
Molecular and Cellular Proteomics, 3(10), mcp-M114 (2015)
Jason A Wojcechowskyj et al.
PloS one, 6(9), e24918-e24918 (2011-09-29)
CXCL12 (SDF-1) is a chemokine that binds to and signals through the seven transmembrane receptor CXCR4. The CXCL12/CXCR4 signaling axis has been implicated in both cancer metastases and human immunodeficiency virus type 1 (HIV-1) infection and a more complete understanding
The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
Villen J and Gygi SP
Nature Protocols, 3(10), 1630-1630 (2008)
Mathilde Biola-Clier et al.
Nanomaterials (Basel, Switzerland), 10(2) (2020-01-25)
TiO2 nanoparticles (NPs) are one of the most produced NPs worldwide and are used in many consumer products. Their impact on human health, especially through inhalation, has been studied for more than two decades. TiO2 is known for its strong
Yu Luo et al.
Inflammation, 43(5), 1667-1679 (2020-06-04)
To better understand the acute inflammatory mechanisms, the modulation, and to investigate the key node in predicting inflammatory diseases, high-sensitivity LC-MS/MS-based proteomics and phosphoproteomics approaches were used to identify differential proteins in RAW264.7 macrophages with lipopolysaccharide (LPS). Furthermore, differential proteins
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