Skip to Content
Merck
All Photos(2)

Documents

11636103001

Roche

PCR Master

(50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase), sufficient for 200 reactions, kit of 1 (2 components), suitable for PCR

Synonym(s):

PCR

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106300

usage

sufficient for 200 reactions
(50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase)

Quality Level

feature

dNTPs included
hotstart: no

packaging

kit of 1 (2 components)

manufacturer/tradename

Roche

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

General description

Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9. The 5′ → 3′ DNA polymerase lacks 3′ → 5′ exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.
The PCR Master contains all the reagents required to perform a standard PCR. All that must be added is the template DNA, the primers, and the required amount of water. The PCR Master contains a fixed MgCl2 concentration of 1.5 mM. However, higher concentrations may be achieved by adding additional MgCl2. PCR products generated by PCR Master are 3′-A single-overhang products. Therefore T/A cloning can be applied.
The PCR Master is stable at +2 to +8 °C, allowing immediate PCR without the time-consuming thawing of reagents.

Application

The PCR Master can be used instead of the single reagent Taq DNA Polmerase, or instead of the PCR Core Kit for nearly all simple, routine polymerase chain reaction applications. The only limitation is that the sample volume must not exceed half the total reaction volume. The PCR master mix is used for:
  • Routine PCR
  • RT-PCR
  • Other primer-extension reactions, such as sequencing and labeling

Features and Benefits

  • Reliable reproducible results:
High lot-to-lot consistency.
  • Convenience:
Adding template and primer requires only two pipetting steps.
  • Prevent PCR carryover:
dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.
  • Ready for immediate use:
Master mix can be stored at +2 to +8°C.

Packaging

1 kit containing 2 components.

Quality

Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Kit Components Only

Product No.
Description

  • PCR Master Mix (10 vials) 2x concentrated

  • Water, PCR Grade (5 vials)

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nury N Olaya-Galán et al.
PloS one, 16(9), e0257492-e0257492 (2021-09-22)
Viruses have been implicated in cancer development in both humans and animals. The role of viruses in cancer is typically to initiate cellular transformation through cellular DNA damage, although specific mechanisms remain unknown. Silent and long-term viral infections need to
Y J Borrell et al.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 44(5), 450-462 (2016-06-10)
A Citizen Science approach was implemented in the laboratory practices of Genetics at the University of Oviedo, related with the engaging topic of Food Control. Real samples of food products consumed by students at home (students as samplers) were employed
Cemalettin Bekpen et al.
Genome research, 22(6), 1050-1058 (2012-03-16)
The LRRC37 gene family maps to a complex region of the human genome and has been subjected to multiple rounds of segmental duplication. We investigate the expression and regulation of this gene family in multiple tissues and organisms and show

Articles

Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.

Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.

Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.

Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service