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53571-U

Supelco

Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core PFP HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L43

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

2.7 μm

pore size

90 Å pore size

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

  • Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employs the Ascentis® Express F5, 2.7 μm HPLC Column to enhance the resolution and sensitivity in the analysis of lumacaftor and ivacaftor, critical medications for treating cystic fibrosis. The method provides significant insights into the stability and degradation pathways of these drugs, facilitating improved drug formulation and storage conditions (Ozcan et al., 2023).
  • Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: Utilizing the Ascentis® Express F5, 2.7 μm HPLC Column, this research develops advanced analytical techniques for tasimelteon, a treatment for non-24-hour sleep-wake disorder. The methods ensure accurate quantification and stability assessment, crucial for clinical efficacy and patient safety (Ozcan et al., 2020).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore

Articles

Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.

Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

Protocols

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

Chromatograms

application for HPLCapplication for HPLC

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