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B9041

Sigma-Aldrich

Butyl-Sepharose 4 Fast Flow

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41106500
NACRES:
NA.56

form

suspension

extent of labeling

~50 μmol per mL gel

matrix

agarose, 4% crosslinked

matrix activation

epoxy

matrix attachment

ether

particle size

45-165 μm

storage temp.

2-8°C

Application

Butyl Sepharose is used in affinity chromatography, hydrophobic interaction media, separation media and resins. Butyl-Sepharose has been used to study inflammatory diseases.

Physical form

Suspension in 20% aqueous ethanol
aqueous ethanol suspension

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

102.2 °F

Flash Point(C)

39 °C


Certificates of Analysis (COA)

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Investigation of protein retention in hydrophobic interaction chromatographic (HIC) systems using the preferential interaction theory and quantitative structure property relationship models.
Chen, J., et al.
Reactive functional Polymers, 67(12), 1561-1569 (2007)
Chemical and chromatographic characterization of a new bioprocess? medium for hydrophobic interaction chromatogrpahy: Butyl Sepharose? 4 FastFlow.
Berggrund, A., et al.
Process. Biochem., 29(6), 455-463 (1994)
Beckley K Nfor et al.
Journal of chromatography. A, 1218(49), 8958-8973 (2011-08-27)
Salt-induced protein precipitation and hydrophobic interaction chromatography (HIC) are two widely used methods for protein purification. In this study, salt effects in protein precipitation and HIC were investigated for a broad combination of proteins, salts and HIC resins. Interrelation between
Wei Yang et al.
Protein expression and purification, 76(1), 59-64 (2010-09-11)
Dipeptidyl peptidase I (DPPI) plays a crucial role in maturation of many regulatory peptides and has been suggested as a pharmaceutical target in several inflammatory diseases. It is also a useful processing enzyme for the generation of authentic protein products
Bridget L Decker et al.
The Journal of biological chemistry, 281(20), 14523-14528 (2006-03-28)
Membrane fusion and protein trafficking to the vacuole are complex processes involving many proteins and lipids. Cytosol from Saccharomyces cerevisiae contains a high Mr activity, which stimulates the in vitro homotypic fusion of isolated yeast vacuoles. Here we purify this

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