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Sigma-Aldrich

Rat Collagen Type I

from rat tail, liquid, 0.05 mg/mL, suitable for cell culture

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

product name

Rat Tail Collagen Coating Solution (20 ML), Type I, 50 μg/mL, solution, BioReagent, suitable for cell culture

Quality Level

sterility

sterile; sterile-filtered

product line

BioReagent

form

solution

shelf life

6 mo.

packaging

pkg of 20 mL

concentration

50 μg/mL

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

General description

Collagen type I is a fibrillar collagen that consists of two α1 chains and one α2 chain twisted together to form a triple helix structure. It is the predominant protein in vertebrates and plays a significant role in the extracellular matrices present in various tissues such as bones, tendons, skin, ligaments, cornea, and other types of connective tissue between cells. Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.
This collagen solution is prepared in 0.02 M Acetic Acid at 50 ug/ml and sterile filtered.

Application

Rat Tail Collagen Coating Solution (20 ML) is suitable for use in cell culture to study the growth, differentiation, and migration of cells. The rat-tail collagen I coating solution has been used to coat 96-well plates, providing a suitable surface for the adhesion and growth of primary keratinocytes in cell culture experiments.

Preparation Note

To prepare the tissue culture ware, apply Rat Tail Collagen Solution at a culture surface ratio of 5 ug/cm2. Here are some examples:
  • For a T-25 flask, use 125 ug (2.5 ml) of the solution.
  • For each well of a 24-well plate, use 10.5 ug (210 ul) of the solution.
After applying the solution, incubate the coated tissue culture ware at 37oC for 2 hours. Carefully remove the Collagen Coating Solution by aspiration. Rinse the coated surface twice with PBS. The coated tissue culture ware can be used immediately or air-dried and stored at 4oC for up to one week.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Mariano Maffei et al.
Scientific reports, 9(1), 19214-19214 (2019-12-18)
Gene delivery using vector or viral-based methods is often limited by technical and safety barriers. A promising alternative that circumvents these shortcomings is the direct delivery of proteins into cells. Here we introduce a non-viral, ligand-mediated protein delivery system capable

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