07-442
Anti-trimethyl-Histone H3 (Lys9) Antibody
Upstate®, from rabbit
Synonym(s):
H3K9me3, Histone H3 (tri methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
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All Photos(5)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat, chicken
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
trimethylation (Lys9)
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Specificity
Expected to cross-react with chicken, mouse and rat.
Trimethyl-histone H3 (Lys9). Broad species cross-reactivity expected.
Immunogen
Epitope: Lys9
KLH-conjugated, 2X-branched synthetic peptide containing the sequence …AR[me3K]ST… in which me3K corresponds to trimethyl-lysine at residue 9 of human histone H3.
Application
Peptide Inhibition:
Specificity was confirmed by the ability of 10 mM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. No inhibition of detection was observed by preabsorption of the antibody with 10 mM histone H3 peptide containing mono- or dimethyl-lysine 9, or mono-, di- or trimethyl-lysine 27 modifications (Data not shown).
Dot Blot Analysis:
Reported by an independent laboratory.
Immunocytochemistry:
Representative lot data. Confocal fluorescent analysis of HeLa cells using anti-Histone pAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue).
Specificity was confirmed by the ability of 10 mM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. No inhibition of detection was observed by preabsorption of the antibody with 10 mM histone H3 peptide containing mono- or dimethyl-lysine 9, or mono-, di- or trimethyl-lysine 27 modifications (Data not shown).
Dot Blot Analysis:
Reported by an independent laboratory.
Immunocytochemistry:
Representative lot data. Confocal fluorescent analysis of HeLa cells using anti-Histone pAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-trimethyl-Histone H3 (Lys9) Antibody (rabbit polyclonal antibody) validated in DB, ICC, Mplex, PIA, WB, ChIP-seq to detect trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9).
Quality
Routinely evaluated by Western Blot on HeLa acid extract and recombinant Histone H3 (cat. # 14-411).
Western Blot Analysis:
1:500 dilution of this lot detected trimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.
Western Blot Analysis:
1:500 dilution of this lot detected trimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.
Target description
17 kDa
Physical form
Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
Storage and Stability
1 year at -20°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Acid-extracted proteins from HeLa cells.
Acid-extracted proteins from HeLa cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chromatin immunoprecipitation (ChIP) is a key technique for studying protein-DNA interactions and mapping epigenetic histone modifications on DNA. Current ChIP protocols require extensive sample handling and large cell numbers. We developed a quick and quantitative (Q(2))ChIP assay suitable for histone
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